CertikLabs · Certificate of Analysiscertiklabs.com/v/CL-7F92-A19D
Sample report
CL-7F92-A19D
Semaglutide · 5 mg · Lot L-2401-77
Verified · Pass
All assays within spec · Anchored Apr 28, 2026
Overall confidenceHigh · 99%
Identity
HighMatchPass
Target: Semaglutide (C₁₈₇H₂₉₁N₄₅O₅₉)
LC-MS [M+4H]⁴⁺ at m/z 1029.6 confirms target peptide. No isoform contamination detected.
Purity (HPLC)
High99.4%Pass
Spec: ≥ 98.0% · USP <621>
96spec 98–100100
1.4 pp above minimum. Single dominant peak; impurity sum 0.6%, all individual <0.3%.
Concentration
High100.6%Pass
Spec: 95.0–105.0% of label · LC-MS
90spec 95–105110
Within ±5% acceptance window. ±0.4% analytical uncertainty (n=3, RSD 0.4%).
Endotoxin (LAL)
< 0.5 EU/mg
Limit < 5 EU/mg
Water content (KF)
3.2%
Spec ≤ 6.0%
Appearance
White lyophilate
Conforms
What this means. This sample is the substance it claims to be (identity), is at least 99% pure (purity), and contains the labeled amount within ±5% (concentration). All three primary acceptance criteria are met with high analytical confidence (RSD < 0.5%, n=3). No reanalysis required.
Method · HPLC-UV
ColumnC18, 4.6 × 150 mm, 3.5 µm
Mobile phase0.1% TFA in water / acetonitrile gradient
Flow1.0 mL/min
DetectionUV 214 nm
Injection10 µL · 1 mg/mL
Method · LC-MS
SourceESI+
Rangem/z 200–2200
CalibrationExternal, ESI tuning mix
Result[M+4H]⁴⁺ confirms target
Content100.6 ± 0.4% of label
Endotoxin · LAL
MethodKinetic chromogenic
Limit< 5 EU/mg (USP)
Result< 0.5 EU/mg (PASS)
Sample chain of custody
ReceivedApr 24, 2026 · 09:14 UTC
LabAurum Bio · ISO 17025 #ABL-2241
AnalystC. Mendes
Reviewed byDr. R. Halberg
Transparency & data sources
Every result is auditableEach headline result links to the originating method, instrument run, raw data file, standard operating procedure, and reference standard used for comparison. Files are content-addressed (SHA-256) and anchored on-chain — tampering breaks the link.
Identity
LC-MS · ESI+ deconvolution
- Instrument
- Waters Xevo G2-XS QTof
- Source
- ESI+, capillary 2.5 kV
- Mass range
- m/z 200–2200
- Reference
- USP Semaglutide RS Lot R094H0
Purity (HPLC)
RP-HPLC-UV · gradient elution
- Column
- C18, 4.6 × 150 mm, 3.5 µm
- Mobile phase
- 0.1% TFA / MeCN gradient
- Detection
- UV 214 nm
- Compendial
- USP <621> system suitability met
Concentration
LC-MS quantitation · external std
- Standard curve
- 5-point, r² = 0.9998
- Replicates
- n = 3, RSD 0.4%
- Acceptance
- 95.0–105.0% of label
- Reference
- USP Semaglutide RS Lot R094H0
All linked artifacts are signed by lab key ABL-2241-KEY-04 and anchored together.
Signature & anchor
Document SHA-256
9a31f8b14c0d…b4f2c0e7
Anchor tx
0x55ce10aa…917b · Apr 28, 2026
Signed by
CertikLabs · CL-SIGN-2026-04
Re-verify
certiklabs.com/anchor/CL-7F92-A19D